fastgroupII

FastGroupII is an online tool to investigate the biodiversity of microbial communities. FastGroupII trims the DNA/RNA sequences with the user specified parameters. Then it deprelicates the sequences and groups the 'similar' sequences together. At the end, you can get the dereplicating and grouping information of your sequences as well as statistical estimation of the community under study.

For help on how to use this tool, please go to fasthelp.

Upload your fasta file

Paste your fasta data

The sequences will be read in random order.

Trim 5' end without N in the first base pairs

Trim 3' end without N in the first base pairs

Trim 5' end with of % similarity

Trim 3' end with of % similarity

Sequences should be at least base pairs long

PSI (Percent Sequence Identity) of % similarity

PSI (Percent Sequence Identity with gaps) of % similarity

Sequence Match of % similarity

Trim off the primers only

General group information

Representative sequences (FASTA format)

Detailed group information

Group statistics

Richness (Chao1)

Diversity (Shannon-Wiener index)

Rank-abundance curve

Rarefaction calculations

text format

plot

All trimmed sequences (FASTA format)